FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052013
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAT69750.06.7956651
CTTCAAT75600.06.55918551
CCCACGT14655.0664623E-46.47095781
CTCAAAT44650.06.2061881
CTCAAGG36701.4551915E-116.15968471
CTCAGTT20652.4225092E-56.00332261
CTCAAAG47600.05.97476051
CTTCGAT50200.05.9558391
CTCAAGA30751.2567398E-85.9286761
CTCTAAA24257.846511E-65.71353241
CTTCAAC61300.05.5911381
CTCTGAT40652.1464075E-105.5611421
CCCTTAA21102.1171987E-45.52968451
CTTAAGT18500.00136323845.51847551
CTTAAAT49801.8189894E-125.41795251
CTTCAGA47751.8189894E-115.34512041
CTCAATA45051.07320375E-105.341731
CCCAAAT34151.2297642E-75.33841231
CCCAGAT42657.6397555E-105.3003611
CCCAGTT35806.094524E-85.2960631
Produced by FastQC (version 0.11.5)