FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592312
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA3605.373451E-516.0819341
CCAAGAT4301.4398605E-515.1469391
CTCAAAT4401.7895234E-514.80269051
CTAGTAC600.00458048614.55864115-119
CTTCCCT3300.008939967513.1579481
CTCAATA4100.002605401212.3556331
CTCCTGT5902.0519068E-512.2658831
CTTATCG5250.001307456511.0276121
CCTTGTT6003.2583665E-410.8553061
TCAGATT6650.0094547368.6809932
CTTTAAA9602.9857512E-48.2922481
CCTCTTT9602.9857512E-48.2922481
ATCAGAT8200.00563176677.9429071
GTTTTCA10256.2369404E-47.7427725
CATTTAT9600.00272831327.5384071
CTTCTTT17401.42619265E-56.6545931
TTCATTT14806.492146E-46.3373733
CTTTCTT16002.3303283E-46.3322621
AGATTTT13750.00196887346.29660134
TTTATCA16850.0030830955.5644777
Produced by FastQC (version 0.11.5)