FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772152
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACT4000.00220787812.6320152
CTCAGAA7451.3030422E-611.7754821
GTCTCTG5109.430877E-411.4676271
CGACCAC4500.00533162811.2277318
CTCTGCT8305.50017E-59.6887641
CTCCATT8004.059257E-49.1382651
CTTCCGA6600.0079942338.8613471
GTATAAT8900.00117262148.2141711
CAAAGAT16457.795643E-87.9994531
CAAGACA8550.0083858157.5982044
CAACGAA8700.0087437877.5627021
GTATGAA8750.0092026977.51948641
CTTTCCC8750.0092026977.51948641
CTTTTGT12103.9681265E-47.25019361
CTTATTT12254.5703372E-47.1614161
CTTTAAT10450.00565679566.9958011
CTTCTCG12556.027787E-46.99022671
CTTTGAT13752.2582288E-46.91185141
CTCAGCA10650.00679771146.8644241
CTTCTCT14303.6478208E-46.6460111