FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066022
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4955.5309156E-613.93283757
CACGTCT5158.327928E-613.3917569
AGCACAC5802.8252934E-511.8909565
ACACGTC5853.0846204E-511.7893248
CACAGAC5200.001728051210.6664251
GAGCACA6751.3219766E-410.2174144
TAACGAT5450.002667417310.1236769
GTCACGT6459.423733E-49.6741991
AGAGCAC7755.2956474E-48.8990383
GCACACG9404.00486E-48.070676
TTCGTGA8800.00186564777.837223
AGTTTGC9600.00437294977.18411875
CCCCGAA14055.615261E-46.4150381
AATTTCT12550.0084629986.04496345
CCCGGAT15000.00123654716.0087531
CTTTCGA14150.0042460655.87972551
AGTCACT3350.00473618655.367912320-24
Produced by FastQC (version 0.11.5)