FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709323
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2353.8198777E-1133.54379
ACACGTC2951.8189894E-1129.150468
CACACGT2853.6925485E-1027.6588427
AGCACAC4101.0841177E-920.9726315
GCACACG4604.5401976E-918.6929996
AGAGCAC5851.408016E-613.473873
GAGCACA6655.916987E-611.8529544
GTTGGAT4958.556751E-411.6012931
AAGAGCA12951.3771425E-47.19534442
CAAAAGA10100.00485083467.10722731
AGTCACT2455.7631574E-47.0196120-24
CAGTCAC3008.3297916E-46.210404420-24
GTCACTC2850.00318453656.03440120-24
CCAGTCA4000.0045078655.016095620-24
Produced by FastQC (version 0.11.5)