FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3218749
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGT14901.5083388E-67.74050431
CTCACGA16005.3703843E-77.6588671
CTTAAAT28900.07.48271041
CCCATGT18456.7758447E-77.03253131
CTTTAAT40400.06.9585511
GTTAGTT10400.00620303266.9311011
CTTTAAA45800.06.76765971
CTCAGTT16304.3875218E-56.63344571
CTCTAAT14203.989428E-46.5991891
CTCGGTT14354.5336105E-46.53020761
CCCCAAT15651.8510317E-46.448361
CTTAAAC14555.363638E-46.44044541
CTCAACA30352.8012437E-106.4126961
CTCCTGA31701.200533E-106.36699151
CTAAAGA22704.634221E-76.3509651
CCCACAG18202.945883E-56.3370061
CTCACAA17309.994141E-56.25001051
CCCAAGA16403.391485E-46.15346531
CTCAGAA27054.4130502E-86.12909171
CAAACGG31103.2905518E-96.0262691
Produced by FastQC (version 0.11.5)