FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322249
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG5551.0893402E-513.0479961
GTCCAAT7301.7819749E-49.9200521
CACGTAC6250.0057589589.2233779
CCCAAAT10701.1207027E-58.7982511
CTACGAT9151.7682256E-48.7057931
GTTGGAT10559.081732E-58.2369331
GTCCAAA8950.00136278938.0912141
TTACTGC9854.135035E-48.0470468
CCTCGAA9150.0016947737.91435771
CTTAAAT10558.1784313E-47.5505221
CTTCGGA16951.2425553E-67.26299861
CTCAAAG12150.00363791276.5562151
CTCTTCT18957.1365776E-66.49645571
CTTCAAT24854.913636E-86.4111071
GGTTGAT13700.0018173826.343041
GTGGAAT14250.0028272866.0982211
CTTCGAT15550.00113045666.05410151
CTAAAAA18050.00108778475.6167821
CTTTACA15500.0071989765.6064291
CTTCCAC20701.77407E-45.5974011
Produced by FastQC (version 0.11.5)