FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361162
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT31700.07.9548641
GGTGTGT18509.112773E-87.3995591
GGTAAAC37000.07.3995591
GGTAGCT17101.5522583E-67.16270071
GGTGTAG28307.2759576E-127.12845751
CTAAGAT19151.2652417E-66.77216771
GGTAAAT47200.06.41108131
GGTGTCT18102.7381177E-56.3689141
GGTAACA30251.14232535E-85.95440771
GTGTAAG15750.00140329395.9468471
GGTGTTT41451.8189894E-125.9098761
GGTATAT46600.05.87518641
GGTATTT65050.05.8701951
GGTAGAA38356.184564E-115.82398561
GGTATAC23857.2630173E-65.73970031
GGTATAG26651.3475765E-65.67735531
GGTGTTC35652.1573214E-95.6587761
GGTATGT26801.4948619E-65.6455791
GGTGATT43951.2732926E-115.5737051
GTGAGAG15550.0079017335.56000041
Produced by FastQC (version 0.11.5)