FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166786
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAA2500.001730030916.8469269
AATCTTG12700.00109706976.6326485
TCCCAGA10600.0095812646.6253422
TCAATCT13854.0511688E-46.58987863
GCAGTTT14507.0677005E-46.2944713
CTTTTGA17057.1934337E-45.8021471
TTGAGCA16100.00248419685.6679619
CTTTGAC16450.0029641265.584221