FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871477
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3059.567884E-1025.4863647
ACACGTC3708.929419E-921.0090278
CACGTCT4651.2250894E-716.7168629
AGCACAC6452.2231688E-814.2445425
GCACACG6707.515304E-611.6020016
AGAGCAC7502.1145916E-611.311873
GAGCACA7753.1345826E-610.9469714
GTTGGTT5400.001934716710.5222731
ATGTAAC5450.002178665310.3730986
GTTTTAT9603.6588547E-48.1383211
GAAGAGC11804.1454678E-47.22291661
CCTTTAT11250.0019845586.94470071
AAGAGCA13954.0211476E-46.59337472
AGTCACG3850.00321807885.15382920-24
ACGTCTG5001.2637593E-45.08806310-14
Produced by FastQC (version 0.11.5)