FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832961
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTT4700.00667611210.8948061
CTTAGAA6556.639815E-410.051261
CTCAATT12659.859523E-78.6740121
CCCATGA13402.2514287E-68.1885271
CCCAAAC11251.7050827E-47.80275631
CTTCCGT25401.8189894E-127.77587651
CTCCCAT14607.618335E-67.5154971
CTCAAAT17853.0556112E-77.37655451
CTCATAT14304.6864807E-57.161621
CTTCGGT18656.339669E-77.06013441
CTTGAAT17902.505034E-66.94728661
CAACTGA12958.5780484E-46.77845571
CTCCATT22002.0125299E-76.6500761
CTTTGAT31306.002665E-116.54384471
CTTGATT21751.163944E-66.3901881
CTTCCGC28051.0411895E-86.25889541
CTCTCTT15207.614196E-46.2563221
ATCAAGT16402.8030056E-46.24458361
CCTCAAT14100.0022437246.2256031
CTCGTTT13050.00689895266.16597131
Produced by FastQC (version 0.11.5)