FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827432
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAG2050.00872728917.8202741
CCCATGT5501.330303E-411.9557831
TCCTAAA4600.00627312710.9852689
CGAAAGG6959.9016965E-510.5126791
GTTCTAT6050.0039263829.6612391
TTGTAGT6050.00433676229.545649
CCATGTC7400.00227911588.7807932
CCTCGAT7750.0031096388.484751
CCGCATT8950.0012469048.1634771
CAATTAT10105.2938465E-47.86310863
ATATACC8400.0071538957.73452576
CCCGCAT8650.00835008557.6019431
CCCGGAG8700.0087912017.55825421
CTCCAGA8800.0097349687.4723641
CAAAGGT10050.00389265647.26996231
CTTCATT12550.00464530856.4039391
CTTCCGG18000.00577289055.27678061
Produced by FastQC (version 0.11.5)