FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759211
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGGT4600.006533879310.9263847
CTCAGAC6300.00608721059.16171551
CCCGCGT7250.00190612778.95636751
CCCATAC11452.7554162E-58.1915341
ATGTCCC9900.0039904157.2525166
CCCGAAA18153.863859E-66.7577121
CTTTAAC14153.7765445E-46.6284851
CTCAAAT14555.30064E-46.4462591
CTTCGAT21701.4178058E-66.3171511
GAAGTAG15159.162422E-46.1610485
GGTCAAT14250.00294854596.0756641
GTTCGAT13200.0089570376.01237631
CTCAAAG18151.9030493E-45.9626871
CAACTGT14750.0043318275.86970951
TTCGATA21204.5310004E-55.7616342
AATGTCC17759.80769E-45.66309175
CTTCAAA32053.907371E-85.62780861
ATTTCGC15400.00736736555.59495747
CTCCAGA18400.00145488735.48956061
CTTGAAT18100.00715303335.18193671
Produced by FastQC (version 0.11.5)