FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889095
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGA4856.682614E-411.9487141
CCCAAAT5702.0092409E-411.4377481
CATGCAT4600.00611431411.0233371
GTCAGAT5350.001519391910.8320111
CAGACAT5650.002392541610.256861
GACATAG5750.002871546410.0337025
AATCCTG8609.559073E-48.3857255
CTCAAAA8700.00102567748.326331
AGATATA8100.00520677458.0134764
GAATCTG8250.00613940657.8677774
CTTCGAA9300.00198124817.78914741
CACCGGA10000.0040321217.24390751
CCCGGAT11700.00243776486.8105111
CTCGCGG12800.0062382816.2252331
CTTTTTT22907.392E-45.06124541
Produced by FastQC (version 0.11.5)