FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791358
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAG8456.4682135E-610.3059331
CTTAACT13401.807283E-47.0404651
CTCAAAT30253.6379788E-126.95721441
CCCAGAG14707.4876545E-56.91151861
CTTCGAA24201.9460094E-76.2974791
CTTTAAT51400.05.92992161
CCTCGAA19859.434782E-55.84954741
CCCATAT29854.623871E-85.83485031
CCCACCT17708.2689134E-45.7400751
CTTCGCT30708.342431E-85.6732991
CTCAAGG23152.4545512E-55.64266541
CCCAGTT31301.2507371E-75.56454561
CTTATAG20901.9792993E-45.55567031
CTCCGCT30153.460991E-75.53609281
CTCAGGA21052.1923793E-45.5160821
CTCTAAT15800.0086973895.51171731
CTTCAAA72400.05.41275071
CCCAGGT17550.00465571555.37562561
CTCATGT17550.00465571555.37562561
CTCAAAA50603.6379788E-125.30657151
Produced by FastQC (version 0.11.5)