FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832644
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGT4001.2121252E-414.6208761
CTCAAAG8554.3730324E-811.9703081
CTTCGTT7251.2385275E-511.0916991
CCCAAAT5400.001521223910.8302771
CTCCGGT7552.2718665E-49.6826991
ATTTACG6200.0047835239.4328221
GTTAAAA7950.00389449728.2759671
CTTCTTA8050.0043590228.173161
TTAAAAC10205.8356236E-47.791942
CTCTTCT9750.00287912027.49788481
CTTTCAT15652.5419886E-67.473931
CTTCATT11953.4403242E-47.34102541
CAGATTC11700.0025027866.79338173
TCAAAGA13852.8505025E-46.78182652
CTTTCAA15109.6338175E-56.77788931
CTTTTAT17308.2013714E-56.3385291
CTTTATT16252.503273E-46.2982231
CTTTTAA14100.00225555526.2216491
CTTTAAA13200.00780526186.09203151
CTTATTT13500.0098457255.95665261
Produced by FastQC (version 0.11.5)