FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564942
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT2050.00912135217.6830021
CTCATAT3558.6807326E-414.2958361
CCCCCAT3050.005243322314.2623561
TTTAAGC3200.00742598413.532432
CCCGCGA3850.001601570613.1818741
CCCCGAT4400.00435921311.5341411
CCTGAAC5350.001507238710.8411671
AATCTAG950.00752968310.720104130-134
CTCCCCG4850.00899410.4639631
CCTTGAA7251.6395179E-410.0000441
GTCTTTA6100.004475779.5082381
CTTATAA6550.0080358538.85500051
CATCAAT9904.2439864E-58.7879161
CTTTCGT6700.0096721758.6567541
CTTGTTT8950.00134431488.1005931
ATCCTGA8250.00585058667.90912441
CTTTCAG12255.0203834E-47.10207131
CCTTCTT10400.0058479156.9711841
CTTTATT12700.0056907136.27955441
Produced by FastQC (version 0.11.5)