FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785965
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGA8901.040351E-610.6019761
CAAGCAC9800.00325096587.40640351
GTTAAGG12000.00311951436.65341851
CCCCATT13650.00170048266.38090131
CTCAAAT15309.053754E-46.1671621
CTCCGAT14550.00348010995.98620651
CCCAGAT14800.0042076555.8850881
CTTTCAA22251.2929442E-55.8718631
CTTCAGG19705.331951E-45.5266051
ACAATCT17550.00472918945.36805733
GGGGGGG3650.0075860755.156549100-104
CTTAAAG18650.0094557675.0593871
Produced by FastQC (version 0.11.5)