FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783749
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGA6254.359115E-410.521131
CCCCACT6450.00665920239.0621281
CTCACGG9100.00147141548.0289451
CCCAGTT13151.3173444E-47.22299771
CTCGGCT10550.0062490076.92544171
CTCATAT17851.8149318E-56.54911
CAAACAC12950.0064489836.20615771
CCCAGAT13000.00671214336.1822881
CCCAGCT14800.00390947565.924061
ACAAACC14700.00413895545.8938013
CATATAC20101.0236821E-45.81599241
GGTAAAG23956.1406918E-65.7962621
CTTAAAT16500.0020676415.75651071
CAACGAA15450.00629966855.6748271
ATTTCGC15300.0064549415.66235456
AAGCGTT16950.00328029065.5370819
AAAGATC15800.0091955945.4834742
CTTAAAA22751.0244919E-45.45968341
CTGAATT19150.00205372325.341451
CTTCGAT18050.0059755665.2621841