FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457625
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGTATTGGCATCTCGTATGCCGT10290.22485659655831738TruSeq Adapter, Index 21 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3250.048.9354637
CACGTCT3700.046.565849
AGAGCAC3950.040.2677573
AGCACAC4150.038.3229525
ACACGTC4550.034.9539038
GCACACG4850.032.7918056
TTTAGGG850.00425423531.187773
GAGCACA5250.030.2966924
GAAGAGC7450.022.3029691
AAGAGCA7700.021.52932
CCCAAAA1900.009712880517.4902231
TCACGTA3300.010.84365425-29
AGTCACG3400.010.52472320-24
ACGTCTG3750.09.89583810-14
CACGTAT3900.09.51522925-29
CAGTCAC4050.09.49005720-24
ACGTATT3800.09.41685725-29
GTCACGT4100.09.37432420-24
AAAAAAA15650.09.14708460-64
ACTCCAG4200.08.83556915-19
Produced by FastQC (version 0.11.5)