FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680427
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCT4453.1975418E-413.0401591
CTAAAAT7101.3217842E-410.2163221
CTTAAAT6405.7887734E-410.2003591
GGAAGTC6300.0059383939.1878612
TTTTACG7954.2449578E-49.0971687
CAACAGA7800.0035170888.3695261
CTTTGGT7800.0035170888.3695261
ACATTTC8400.0070332167.74883754
CTTAAAA9450.00228663427.6757551
CTTTTAA10759.798561E-47.4222771
GGAATTT10500.00657494466.8878553
CAAGAAA15250.0058944845.70774171
TTGATTT18300.0078851975.13766344
Produced by FastQC (version 0.11.5)