FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985567
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA15101.8915307E-78.17517951
CAAACGT11001.4311337E-47.92161751
CTTGCGT10900.00112515367.32810161
ACATGTT10100.00455116857.1553674
CTCGCAG15552.0514693E-57.0046461
CCCGATT13802.5712914E-46.8405271
CTCATCT13050.00101784076.67722561
CCTGGTT12650.0053893786.3143331
GTCCGAT12700.00561521576.28947351
CGTTGAA19751.2999328E-56.250391
CAAACGA13400.0097896665.9609191
TCAATGT19507.7391705E-55.92978143
CTTGCAA21554.9508664E-55.72831541
CTTCCGT23152.4274603E-55.6460771
CAAAACT15400.0068595185.63136673
CCCCGAC16850.00285571725.6023311
CTGTAAT17300.0039005765.4566061
CAGGTTT18700.00174744755.4105293
CCCGTTT20509.0459076E-45.313281
ATTTCGC23251.675268E-45.2840676
Produced by FastQC (version 0.11.5)