FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595933
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC2253.1994205E-828.6184279
TTCCCTA2853.1528725E-722.5934948
ACACTCT3209.1438596E-720.2258531
CACTCTT4854.9325165E-513.2922042
ACTCTTT6851.05455954E-410.4464343
CTTTCCC8951.3364988E-610.393916
TTTCCCT9101.657756E-610.2208667
CTATAAT6456.7280245E-410.0345321
CTCTTTC9900.00411421157.22808844
TCTTTCC12550.00576358856.2720225
CCTACAC3550.00102677645.643069710-14
TCCGATC3550.0062243575.24421825-29
Produced by FastQC (version 0.11.5)