FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097877
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCT4550.005219137811.2605781
CCCCAAT4550.005219137811.2605781
CCCAGTT5952.7189488E-411.0713231
CTCCAAG6656.2166684E-511.0065791
CTCAGAT5600.00203942710.456251
CTCCGAA6150.00443157839.5211391
CCCATCT7050.00129489589.34388351
TTCGATT12156.6120265E-78.9169022
CTTGTGA6650.0084258128.80526351
CTCCAAT9201.6700318E-48.7514271
CTTCGAT10901.2365974E-58.729531
CTCAAGG6750.0095205048.6748151
CTCCTCT8000.0040780018.2342971
CTTTCTT28450.08.232691
CTTTCAT23753.6379788E-128.012791
CTCCATT12901.1827695E-57.9435081
CTTATAT16104.6326386E-77.72853331
CTTAAAG10758.912659E-47.48959351
CCCATTT10809.3643425E-47.454921
CAACAAA19802.8885552E-87.3933081
Produced by FastQC (version 0.11.5)