FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087328
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACT10951.2495355E-58.7234261
CCCAAGT8809.988796E-48.3497821
GCCTTAA10150.00589628567.9041147145
CCCACTT11251.619453E-47.83766271
CCCACAT13802.2247985E-46.92184931
CTCCGTA13400.0012017536.5801271
CTTGCAT14554.246902E-46.56505251
CCCAGCT19651.0011183E-56.35688261
CCCGGTT17751.09228735E-46.2094161
CTCAATT14300.0024999526.1659931
CTCCAAC16000.00133806585.9700941
GTCAAAT21203.2208016E-55.8921111
CTCAAAT19052.8698397E-45.7856771
CTCCGAT16550.00200425345.77169231
TAAAGCG15050.0054461985.7500623
CCTCAAG15550.006356465.6703351
CTTCGTA17000.00275749645.61891271
CCCATTT19704.520697E-45.5947781
CTTTTAT39906.2755134E-105.52466871
CTTCAAT38801.7989805E-95.4919191
Produced by FastQC (version 0.11.5)