FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472301
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC8300.00622783977.85592461
GTTGAAT12000.00317946736.6411661
CTTAAAT10950.0097108166.61635451
GAATAAC12300.00433060576.44794136
TTGAATT20403.5071062E-66.36325172
CTTGAAT13900.00212883486.25459771
CTTTCGA12950.0070374916.1539761
CTTGCTT14300.00292611476.07964371
CCCGCTT14750.00413473375.8941631
CGAATAT16000.00158325265.88648841
GTTTGAA17700.0052416115.32111931
TATTTGC17700.00554557665.2956395
GGGGGGG3850.00232053555.2903996120-124
CTTTTTT48503.45608E-115.22828441
CCAAAAT18100.0068113175.2035261
CGCTGAT19850.00357621445.10975931
Produced by FastQC (version 0.11.5)