FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074502
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC10700.0079210646.75823269
TTCCCTA14906.884701E-46.3091958
ACACTCT16550.00237773135.6898171
CTCGGGA15300.00622699645.68123531
CTCGCGT15300.00622699645.68123531
CCCCCAT15900.0095156755.4668491
CCCGAAT21202.4919445E-45.4668491
CTCAGAA24353.2288182E-45.05711561
GTTTTAG21700.00201285355.0033895
Produced by FastQC (version 0.11.5)