FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735438
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT20708.944153E-86.95391041
CCCCCTT18951.0826043E-66.8364831
CTCAATT25401.7571438E-96.80059581
CCTAAAA25951.9468644E-86.3791071
CCTAAAG13950.00238990326.1912231
CTTTTAA61900.06.1624681
CCCCTTT36451.6370905E-116.1211581
CTTAATT23701.0334861E-66.07366851
CCTAATA13100.008498386.0435321
CTCATTT44250.06.0180791
CCCAGAT49450.05.96742531
CCCACGT14500.00368306835.9563841
CTCAAAT37603.8198777E-115.93394231
CTTTCGA40659.094947E-125.84282451
CTTCAAT63100.05.817151
CCCAAGA22752.1205477E-55.6945651
CCCATAT36958.931238E-105.64875841
CCCCTCT17950.00109809865.6134911
CTCCGTT43601.0913936E-115.6125711
CTTCCAT77600.05.56492041
Produced by FastQC (version 0.11.5)