FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676444
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT5150.001173261811.1718961
CTTCGGT6150.00511800039.3553281
CTCAAAG7703.2601383E-49.340141
CTTTAAG7400.00235932468.7469151
CTTAAAA10156.253207E-58.50274851
CACAAAT7900.0042613568.1933131
CAATGAT10701.1573103E-48.0656911
CACAAAG8400.0073942247.70561551
CTTTCAT13101.8312103E-57.68600851
CCTTCAT9400.00236037547.6509661
CCCTTTT8500.0082196937.6149611
CTTTAAT9950.0041161447.2280481
CTTCAAT12155.0163287E-47.1031191
CTTCCTT12657.948978E-46.8223631
CAAAAGA15701.978386E-46.4131661
CTTTATT14957.6274807E-46.25383231
TTTATTC14250.00308575236.04962542
ATTTTAT24401.1104074E-55.59404376
TCTTTAT18450.0093762995.0618473
Produced by FastQC (version 0.11.5)