FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549883
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAG2800.00294175815.5573081
CACGGTT3800.001475232413.3263443
CTTCAAG9701.3096724E-1012.7238461
CTTAAAT5259.132862E-512.4458451
CTCAAAC4957.77624E-411.73345851
ACGATTA4550.005687942711.1296943
CTTTAAT6355.337801E-410.2898731
CTATAAA5850.00313078299.928311
TAAAGCC6050.00425724969.5660194
CCAAAGA7450.00230078148.7705621
TTTAATT10004.6398546E-47.95918042
ATCAGAT9450.00226494797.6826211
AAGATTT11050.00134920227.2015673
AAAGATT11800.00269374116.7450682
CAAAGAT11950.00296878746.68291571
CTTGAAA12700.0056086756.28825571
AAATATC13950.00224598946.22305544
CTTTTTT20851.8874812E-45.5712821
Produced by FastQC (version 0.11.5)