FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002111
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4305.3445547E-817.9774079
CACACGT4004.9387927E-717.5697087
ACACGTC4259.3189556E-716.535378
AGAGCAC7101.5197693E-510.8893563
GAGCACA6608.6905115E-510.649374
CTCAGAT4750.00940339810.4025581
CCCCCCC950.0097204410.35739780-84
GCACACG6801.1771987E-410.3351226
AGCACAC7101.8197177E-49.898925
CTTAAAT8005.764142E-48.8235981
CTGGCGT6450.0088274968.7551981
CCCAGAT7450.00297258128.5275041
CCCAGCT8550.0011158848.2559981
CCTCGAT9453.3013977E-48.2166831
TCAGCCG10450.00101618547.3977726
CCCTGCT12901.5875287E-47.1135981
CTCGATG10300.00714804656.8293212
AAGAGCA13800.00273130536.11669642
CCAGAAT14250.00375899325.9443191
CTTCATA14900.0061687235.6850031
Produced by FastQC (version 0.11.5)