FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113438
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAG2850.003447751515.1938596
CCCACAC10255.92861E-47.7817991
CCAACTA8350.0068011367.77944374
TTAGCAT10000.004186337.2170838
ATTAGCA10500.0067238126.87341177
GTCACAG11850.0027622376.73109251
CTCAAAT15251.2225704E-46.65685941
CACATCT15600.0080228755.5519963
CACGATC19655.535004E-45.51144032
GTTAAAA21502.9956014E-45.3962581
GTTTGAT23251.5926966E-45.30196861
AGATTCA21100.00144198895.13062766
CTTTCGA18500.0087076675.09545331
Produced by FastQC (version 0.11.5)