FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520393
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTA400.00794831950.747454
TCCTAGT400.00794831950.747453
GGTCCAT1350.001163835325.2228721
GGACCAT1700.004610797419.9009635
GTTCGAA3150.009819644512.9717621
CAAATTA4150.00471548711.4130823
CGAATGA3800.007239704510.15476145
GCTCGTC5650.00417999139.5870892
CGACGAA5750.00145786168.947963145
CAAAAAT11100.00267728446.7488221
GTCACGA4100.00146569635.924803130-134
CACGACG4950.00307002035.568088140-144
CTCCAGT4550.00150474255.4750414125-129
GACGAAT5150.00205144365.341936135-139
Produced by FastQC (version 0.11.5)