FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719829
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT8053.3552042E-610.8866831
CTACATT14508.981442E-88.5623141
CCCAAAG10305.784585E-47.799481
CTTGGAT10850.00100800357.4041151
CTTTAAT25702.910383E-117.38840061
CTCATCT15652.0705718E-56.9998241
CTCCGCT14757.196069E-56.9318031
TATTGGC11500.00212383766.9011157
CCCCTTT10700.00719421546.82537361
CTCAATT10750.00752589676.79362771
CTTCGAT15109.784236E-56.77113151
CTTCAAA29801.3096724E-106.6169481
CTTGCAT14554.5722642E-46.5251511
TTCGATT15751.985255E-46.41315752
CTCTTGA15951.996757E-46.41028831
CTTCATT26702.5800546E-86.2911031
CTCAAAA24402.0116386E-76.2854971
CCCATTT18603.3397635E-56.2822791
GTCTTCT13200.0078980376.0859581
CCCATAT13200.0078980376.0859581
Produced by FastQC (version 0.11.5)