FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327229
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT11402.9912553E-79.4198581
CCTCGAT11300.00191453346.96902851
CCCAAAA13702.7964034E-46.7932991
CTCAAAA18807.5375756E-66.47364761
CTGACAA13650.00198433736.29370741
CTTCATT21606.3545995E-55.6344711
CCGCTCC15300.0072915945.5999463
CAAAAAT34704.551839E-85.3641611
CTTTCAA20650.00120381555.20030931
CTTCCAT21300.00181647435.04161451
Produced by FastQC (version 0.11.5)