FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423070
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG11201.6971171E-810.3736171
CTCCGAT14551.03682396E-109.9814861
CTCTCAG6150.0047327759.4458941
CTGGGAT8608.74461E-59.2880061
CCCAAAA11001.5386031E-69.2419491
CTTACGA7700.0031034018.4875041
CCCCTTT8800.00112231968.251741
CCTCTAT9000.0014015438.0683691
CTCAAAC11703.3292505E-58.0683681
CTAAAAT11853.9038547E-57.9662371
CTTCGAT14609.911673E-77.9578431
CTTATTC10406.81702E-47.6804661
CTCAAGG14306.296932E-67.6169911
CTCAGAA8600.0083838577.5992781
CCTCAAT11702.91843E-47.44772431
GATTCGG9900.00374673177.29931457
CCCCGGT9950.0037531847.2980221
GGATTTA10900.00118455297.29236134
CTTCAAA25251.6916601E-107.18963531
TCCGATT14104.5460005E-57.1778452
Produced by FastQC (version 0.11.5)