FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114293
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT18257.146082E-87.5017991
CACGAAT12800.0066009896.1923771
TCCTTAA13250.0097199695.9650945
CGAACGA16400.00226940425.7118161
CTCCGAT16550.00252886075.6600471
GTTAAAA18500.00158374345.45294331
CTTCTAC18650.0017535175.40908581
TAAATCT18700.00187583585.3802043
CTTTGAG21653.6164286E-45.325211
CAAATTT19900.00408478555.055773
CTTTAAT22857.744425E-45.04554941