FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167404
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5957.1757866E-519.818117145
TCAGACG11704.0017767E-1114.397778145
TCTCGTA7550.003236878413.387073145
GAGCGGA10100.00397924911.67503145
CAGTCAC9051.472963E-711.1682205145
AAATAGA10950.007264759410.768748145
CTCAGAC8500.09.477823140-144
CACTCAG7400.09.385078140-144
ACTCAGA8050.09.317458140-144
CGAATCT6805.020411E-108.579065140-144
CACGTCT7607.953326E-48.5430379
CAGACGA11952.0003972E-78.457941145
AATCTCG9251.8189894E-128.108706140-144
GAATCTC9451.8189894E-127.937094140-144
ATTAATA9256.408528E-47.72469762
AGTCACT6854.7475623E-97.6471705135-139
AGAGCAC8700.00301253287.4638423
ATCTCGT9706.9469024E-86.9465566145
TCACTCA8151.0576514E-76.8171363140-144
CACACGT7701.7418222E-56.74567037
Produced by FastQC (version 0.11.5)