FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587264
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT1500.001415216524.3911511
CTCCCAT4501.9931644E-514.6346891
CAACTTG3050.005352596814.2182284
CTTTAGA3150.00610815513.9377991
CTCCAAA5400.00150812410.8405111
CTCCTGT6807.806122E-510.7608011
TATTGCA7750.00342655978.3933427
CTTCCTT12204.309965E-47.1973881
CAACAAA10850.0080582916.7440971
GTTTTAT11050.0096104346.6220321
Produced by FastQC (version 0.11.5)