FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983485
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCT4957.488944E-411.7879321
TCCAATT6100.0046963069.4542362
CTTCAAG13352.3730172E-78.7416121
AACAACC9150.00177125167.87853052
CTCTTCT12054.5466302E-57.8688111
CTTGTAA10557.5703656E-47.60489231
CCTCAAT8800.0098884197.459551
CTTTCGG14951.1065353E-57.31817631
CTTTCTC14204.4313558E-57.19105341
CTTTAAA16303.712734E-56.712071
CCCATTT10950.0090952836.66098931
CAACAAC13150.00105382016.65592341
CTCAAAA16002.1108016E-46.382061
CTTCCTT13950.00205316196.2742221
CTTTAAT14400.00293130576.0781521
CTTTGAT16000.00145921275.92619851
CTTTATT17758.0155436E-45.7528431
CTTCTTT28154.8694346E-75.7002921
CAAAGAT15450.00641254855.66507431
CTTATTT15700.00765595775.57486531
Produced by FastQC (version 0.11.5)