FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648298
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT8457.1244394E-610.222427
CACGTCT8507.643204E-610.1622889
ACACGTC9803.973335E-69.5487498
GCACACG16155.1380885E-67.13223846
AGAGCAC16052.583991E-46.2843383
AGCACAC15800.0014579265.92767765
CTCAGAA18350.00141803335.50102141
CTCAAAC20057.391923E-45.3942151
CAAACCG21500.00187350275.0304191
Produced by FastQC (version 0.11.5)