FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405441
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCAC11051.614744E-47.8391747
CTCCACA12003.9210098E-47.25886251
CTTAAAT14850.004362745.8657471
CTTTAAT22851.978719E-55.7181411
CTTATGA17150.00353203275.50234461
CTTGATT21452.851662E-45.4145361
CTTCTAT21000.00125252265.18490171
TTTGTTA19500.00299708315.1825658
CTTTTAT36701.1652082E-75.14251851
CCCGGAA18600.0091520715.0733981