FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542274
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC3901.1643514E-414.6883571
CTCCACT2950.004563131414.5638781
CTCAAGA4200.00337810211.934291
GAATGTC4600.006996043510.8241762
CTTCGAT4850.0098594110.3348481
ATTCAAT7400.0024529798.7088061
AACAAAT9650.00318327157.4202841
TTCATCT11100.00169262557.0489492
CACTGGT2650.0086226216.034825105-109
TTCTTCC14600.0045125035.84632643
CAAAAAA17357.5589586E-45.77798461
Produced by FastQC (version 0.11.5)