FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118447
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAC11301.5224941E-910.9889681
CCCAACT7451.6930237E-510.7850681
CTAAATT11903.9717634E-89.8210851
CTAAAAG11402.0218182E-47.68887571
CTCAAAC13502.2310987E-57.57496641
CTCAATT12154.2113598E-47.2142541
CTCATGA13401.6671956E-47.0863891
CCAAGTA11350.00183104366.99898348
CCCAAAT10450.00571330966.98988681
CTCCCGT11650.0021381516.89688831
CTCAAAT18353.7833433E-66.7670491
CACGATT14103.1123593E-46.7345831
CCCCGAT12150.00332566236.61306571
CCAAGAC14404.023503E-46.5942791
CCCAGAT20603.342302E-66.38251351
CTCGAAA14906.0905475E-46.37299441
TGTAACC14906.9913163E-46.30079277
CCCCGAA12800.0057304246.27724651
CTCAAAG19801.2342003E-56.27148251
CTCAGCC15207.751316E-46.2472121
Produced by FastQC (version 0.11.5)