FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352620
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGC6950.00128434429.3526046
CCCAATT7300.00168696149.077061
CTCAAAG12709.4997995E-78.695871
CCCAGCT10355.9855458E-58.5362381
CCCACGG8150.00457377178.1303731
CCTCTAC8350.005687747.9356331
CCCGAAT8350.005687747.9356331
GGTAGAT8450.00632894047.841721
CTCGTTT9450.0019781897.79100941
CCCAGAT12456.081378E-57.68775561
CTCCGTT11502.0392539E-47.6826131
GTTCATT14454.9344697E-57.1332221
CTTTGAA20102.6533417E-76.9595811
CCCGCAA12550.00429317966.4531911
CCACCAT17156.606377E-56.4395081
CCCTGAT12700.0048598146.37697171
CCTTCCT12800.005273626.32715231
CAACAAC19204.7121986E-56.13542031
CACAAAG15050.00432368655.8704351
CTTCAAG21353.4744116E-55.8624161
Produced by FastQC (version 0.11.5)