FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555916
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACT3100.00523461314.2658181
CCCTTGT4251.8904598E-413.8742071
GACAGTA3200.00737950513.5452262
AAGATTG6354.426009E-511.3775925
TCAGATT5852.6192836E-411.1140322
CCCAGAT4850.00795751510.6380831
CGATCAT5650.00206915210.436351
CTTAAAT5000.00997272110.3189411
CGTTCAT5850.00276210610.0795521
ATCGCTG5850.00326229039.8791392
CTTTGAA9300.00166767037.9254531
TCACTCA2350.00263051256.762641415-19
Produced by FastQC (version 0.11.5)