FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656249
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTT6403.0786086E-612.5663121
CCCCATC4250.0031592812.0421541
CACTGAT4550.00525832311.2481671
CTTCGCT4850.00845234710.5524041
ATATTCG7300.00201119048.9019726
CCCTTTT7450.0021595978.8324541
CCTTCAT9350.00190499797.81958151
CTTTATA11500.00184155216.9934261
CTTTGAT10550.00619542466.9301511
TTATTCT11650.00240876086.81762939
CATCAAT12800.00566008046.2831561
Produced by FastQC (version 0.11.5)