FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055785
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGG6854.877511E-712.7732491
CTCTGAT5500.001812208910.6056671
CTAATTT8507.353455E-59.4359241
CCTCGAT8751.00906305E-49.1663261
CACAGAC6400.00634914739.1142441
CTTCGAC8700.0089572057.5428231
CTAAAAA14557.58406E-67.5169031
CTCATCC10809.753276E-47.42642161
CAACGAA11953.5477395E-47.32190371
CTTCTAT12355.175121E-47.08475641
CAATAAT16353.893255E-56.68935351
GAATGTT10850.00932631556.64378934
CTTTCCT15551.4637066E-46.56461
CAACAAA18402.9139488E-56.3403441
CTTCCAA12950.0065813576.19346331
CAATCTG12900.0071187256.1467934
GTTTGTT13250.00834379256.0532341
CTTTGAT15750.00121315796.01829431
ATCAGAT14550.0033039976.0135221
GAACTTC14800.00453738965.8447394
Produced by FastQC (version 0.11.5)