FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645661
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1804.8770034E-628.0581538
CACACGT2151.9356325E-523.4905477
CACGTCT2506.197096E-520.201879
CCTCTCG2850.003436855315.1987922
CTTCCCC5056.470907E-512.9133351
AGAGCAC4503.6709502E-412.8285723
TCACATA4250.003484974411.8852944
GAGCACA5702.0807283E-411.3937974
AGCACAC4450.004918710311.3493655
GCACACG4550.00580435111.0999296
CTCCGAC4850.0090352110.4578781
Produced by FastQC (version 0.11.5)