FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829045
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAT5752.0472387E-411.414491
CCCGTTC6607.315579E-49.9444411
CTCCAAC6200.00488083449.4097941
CTTTTAT16206.9267116E-98.5530381
CTCAAAG7800.00335229128.4145281
AAGACTT8700.0099300617.45577674
CATGGAG9900.00342353577.3662531
TTTAATT14054.505713E-57.1816022
CTTTAAT10700.00728607746.8155051
CAAAGAG10700.00728607746.8155051
CTTCAAA14906.196129E-46.3626631
CTGAAAT13200.0080051556.07715851
CAAAGGA13200.0080051556.07715851
CTTTCAT15450.0064207845.6641481
CTGAAAG17250.00357588085.49586531
Produced by FastQC (version 0.11.5)